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  Secondary Antibody Conjugates for Flow Cytometry
Antibody Description Unconjugated NEW
DyLight
488
A=493, E=518		 Fluorescein
FITC
A=488, E=520		 Phycoerythrin
R-PE
A=488, E=580		 Allophycocyanin
APC
A=633, 635, E=670		 NEW
DyLight
649*
A=652, E=670

NEW
PerCP
A=488, E=675

 Biotin-SP
(long spacer)

  F(ab')2 Fragment Donkey Anti-Chicken IgY†(IgG)(H+L)                  ML*
  (min X Bov, Gt, GP, Sy Hms, Hrs, Hu, Ms, Rb, Rat, Shp Sr Prot)

 € 92 / 0.5 mg
703-006-155		 € 193 / 0.3 mg
703-486-155		 € 138 / 0.3 mg
703-096-155		    € 285 / 1.0 ml
703-116-155		 € 285 / 0.5 ml
703-136-155		 € 193 / 0.3 mg
703-496-155		 € 285 / 0.5 ml
703-126-155		 € 173 / 0.3 ml
703-066-155
  F(ab')2 Fragment Donkey Anti-Goat IgG (H+L)♦                              ML*
  (min X Ck, GP, Sy Hms, Hrs, Hu, Ms, Rb, Rat Sr Prot)		 92 / 0.5
705-006-147		 193 / 0.3
705-486-147		 138 / 0.3
705-096-147		 285 / 1.0
705-116-147		 285 / 0.5
705-136-147		 193 / 0.3
705-496-147		 285 / 0.5
705-126-147		 173 / 0.3
705-066-147
  F(ab')2 Fragment Donkey Anti-Guinea Pig IgG (H+L)                      ML*
  (min X Bov, Ck, Gt, Sy Hms, Hrs, Hu, Ms, Rb, Rat, Shp Sr Prot)		 92 / 0.5
706-006-148		 193 / 0.3
706-486-148		 138 / 0.3
706-096-148		 285 / 1.0
706-116-148		 285 / 0.5
706-136-148		 193 / 0.3
706-496-148		 285 / 0.5
706-126-148		 173 / 0.3
706-066-148
  Whole IgG Goat Anti-Armenian Hamster IgG (H+L)                         ML*
  (min X Bov, Hu, Ms, Rb, Rat Sr Prot)		 160 / 1.0
127-005-160		 227 / 0.5
127-485-160		 164 / 0.5
127-095-160		     285 / 1.0
127-115-160		 285 / 0.5
127-135-160		 227 / 0.5
127-495-160		 285 / 0.5
127-125-160		 204 / 0.5
127-065-160
  F(ab')2 Fragment Donkey Anti-Human IgG (H+L)
  (min X Bov, Ck, Gt, GP, Sy Hms, Hrs, Ms, Rb, Rat, Shp Sr Prot)		 92 / 0.5
709-006-149		 193 / 0.3
709-486-149		 138 / 0.3
709-096-149		 285 / 1.0
709-116-149		 285 / 0.5
709-136-149		 193 / 0.3
709-496-149		 285 / 0.5
709-126-149		 173 / 0.3
709-066-149
  F(ab')2 Fragment Donkey Anti-Human IgG, Fcγ fragment specific
  (min X Bov, Hrs, Ms Sr Prot)		 148 / 1.0
709-006-098		 267 / 1.0
709-486-098		 206 / 1.0
709-096-098		 285 / 1.0
709-116-098		 285 / 0.5
709-136-098		 267 / 1.0
709-496-098		 285 / 0.5
709-126-098		 170 / 0.5
709-066-098
  F(ab')2 Fragment Donkey Anti-Human IgM, Fc5μ fragment specific
  (min X Bov, Hrs Sr Prot)		 206 / 1.0
709-006-073		 267 / 1.0
709-486-073		 229 / 1.0
709-096-073		 285 / 1.0
709-116-073		 285 / 0.5
709-136-073		 267 / 1.0
709-496-073		 285 / 0.5
709-126-073		 170 / 0.5
709-066-073
  F(ab')2 Fragment Goat Anti-Human IgG (H+L)
  (min X Bov, Hrs, Ms Sr Prot)		 116 / 1.0
109-006-088		 203 / 1.0
109-486-088		 156 / 1.0
109-096-088		 285 / 1.0
109-116-088		 285 / 0.5
109-136-088		 203 / 1.0
109-496-088		 285 / 0.5
109-126-088		 130 / 0.5
109-066-088
Antibody Description Unconjugated NEW
DyLight
488
A=493, E=518		 Fluorescein
FITC
A=488, E=520		 Phycoerythrin
R-PE
A=488, E=580		 Allophycocyanin
APC
A=633, 635, E=670		 NEW
DyLight
649*
A=652, E=670

NEW
PerCP
A=488, E=675

 Biotin-SP
(long spacer)
  F(ab')2 Fragment Goat Anti-Human IgG, Fcγ fragment specific
  (min X Bov, Hrs, Ms Sr Prot)		 € 148 / 1.0 mg
109-006-098		 € 267 / 1.0 mg
109-486-098		 € 206 / 1.0 mg
109-096-098		 € 285 / 1.0 ml
109-116-098		 € 285 / 0.5 ml
109-136-098		 € 267 / 1.0 mg
109-496-098		 € 285 / 0.5 ml
109-126-098		 € 170 / 0.5 ml
109-066-098
  Whole IgG Goat Anti-Human IgG, Fcγ fragment specific
  (min X Bov, Hrs, Ms Sr Prot)		 103 / 1.0
109-005-098		 171 / 1.0
109-485-098		 130 / 1.0
109-095-098		 285 / 1.0
109-115-098		 285 / 0.5
109-135-098		 171 / 1.0
109-495-098		 285 / 0.5
109-125-098		 163 / 1.0
109-065-098
  F(ab')2 Fragment Goat Anti-Human IgG, Fcγ fragment specific
  (min X Bov, Ms, Rb Sr Prot)		 148 / 1.0
109-006-170
267 / 1.0
109-486-170
 206 / 1.0
109-096-170		 285 / 1.0
109-116-170		 285 / 0.5
109-136-170		 267 / 1.0
109-496-170		 285 / 0.5
109-126-170		 170 / 0.5
109-066-170
  F(ab')2 Fragment Goat Anti-Human IgG, F(ab')2 fragment specific
  (min X Bov, Hrs, Ms Sr Prot)		 148 / 1.0
109-006-097		 267 / 1.0
109-486-097		 206 / 1.0
109-096-097		 285 / 1.0
109-116-097		 285 / 0.5
109-136-097		 267 / 1.0
109-496-097		 285 / 0.5
109-126-097		 170 / 0.5
109-066-097
  F(ab')2 Fragment Goat Anti-Human IgG + IgM (H+L)
  (min X Bov Sr Prot)		 147 / 1.0
109-006-127		 254 / 1.0
109-486-127		 194 / 1.0
109-096-127		     285 / 1.0
109-116-127		 285 / 0.5
109-136-127		 254 / 1.0
109-496-127		 285 / 0.5
109-126-127		 167 / 0.5
109-066-127
  Whole IgG Goat Anti-Human IgA, alpha chain specific

 113 / 2.0
109-005-011		 180 / 2.0
109-485-011		 137 / 2.0
109-095-011		 285 / 1.0 ml
109-115-011		 285 / 0.5
109-135-011		 180 / 2.0
109-495-011		 285 / 0.5
109-175-011		 171 / 2.0
109-065-011
  F(ab')2 Fragment Donkey Anti-Mouse IgG (H+L)                            ML*
  (min X Bov, Ck, Gt, GP, Sy Hms, Hrs, Hu, Rb, Shp Sr Prot)		 92 / 0.5
715-006-150		 193 / 0.3
715-486-150		 138 / 0.3
715-096-150		 285 / 1.0
715-116-150		 285 / 0.5
715-136-150		 193 / 0.3
715-496-150		 285 / 05
715-126-150		 173 / 0.3
715-066-150
  F(ab')2 Fragment Donkey Anti-Mouse IgG (H+L)**                                 ML*
  (min X Bov, Ck, Gt, GP, Sy Hms, Hrs, Hu, Rb, Rat, Shp Sr Prot)		 191 / 0.5
715-006-151		 276 / 0.3
715-486-151		 197 / 0.3
715-096-151		 285 / 1.0
715-116-151		 285 / 0.5
715-136-151		 276 / 0.3
715-496-151		 285 / 0.5
715-126-151		 234 / 0.3
715-066-151
Antibody Description Unconjugated NEW
DyLight
488
A=493, E=518		 Fluorescein
FITC
A=488, E=520		 Phycoerythrin
R-PE
A=488, E=580		 Allophycocyanin
APC
A=633, 635, E=670		 NEW
DyLight
649*
A=652, E=670

NEW
PerCP
A=488, E=675

 Biotin-SP
(long spacer)
  F(ab')2 Fragment Goat Anti-Mouse IgG (H+L)                                        ML*
  (min X Hu, Bov, Hrs, Rb, Sw Sr Prot)		 € 147 / 1.0 mg
115-006-146		 € 256 / 1.0 mg
115-486-146		 € 194 / 1.0 mg
115-096-146		 € 285 / 1.0 ml
115-116-146		 € 285 / 0.5 ml
115-136-146		 € 256 / 1.0 mg
115-496-146		 € 285 / 0.5 ml
115-126-146		 € 160 / 0.5 ml  
115-066-146
  F(ab')2 Fragment Goat Anti-Mouse IgG Fcγ fragment specific               ML*
  (min X Hu, Bov, Hrs Sr Prot)		 148 / 1.0
115-006-071		 281 / 1.0
115-486-071		 213 / 1.0
115-096-071		 285 / 1.0
115-116-071		 285 / 0.5
115-136-071		 281 / 1.0
115-496-071		 285 / 0.5
115-126-071		 181 / 0.5
115-066-071
  Whole IgG Goat Anti-Mouse IgG (subclasses 1+2a+2b+3),                   ML*
  Fcγ fragment   specific (min X Hu, Bov, Rb Sr Prot) 		 113 / 1.0
115-005-164		 226 / 1.0
115-485-164		 171 / 1.0
115-095-164		     285 / 1.0
115-115-164		 € 285 / 0.5
115-135-164		 226 / 1.0
115-495-164		 285 / 0.5
115-125-164		 194 / 1.0
115-065-164
  Whole IgG Goat Anti-Mouse IgG, Fcγ subclass 1 specific                ML*
  (min X Hu, Bov, Rb Sr Prot)		 200 / 1.0
115-005-205		 284 / 0.5
115-485-205		 214 / 0.5
115-095-205		     285 / 0.5 ml
115-115-205		 285 / 0.3
115-135-205		 284 / 0.5
115-495-205		 285 / 0.3
115-125-205		 256 / 0.5
115-065-205
  Whole IgG Goat Anti-Mouse IgG, Fcγ subclass 2a specific                   ML*
  (min X Hu, Bov, Rb Sr Prot)		 200 / 1.0
115-005-206		 284 / 0.5
115-485-206		 214 / 0.5
115-095-206		     285 / 0.5
115-115-206		 285 / 0.3
115-135-206		 284 / 0.5
115-495-206		 285 / 0.3
115-125-206		 256 / 0.5
115-065-206
  Whole IgG Goat Anti-Mouse IgG, Fcγ subclass 2b specific                   ML*
  (min X Hu, Bov, Rb Sr Prot)                                                             		 200 / 1.0
115-005-207		 284 / 0.5
115-485-207		 214 / 0.5
115-095-207		     285 / 0.5
115-115-207		 285 / 0.3
115-135-207		 284 / 0.5
115-495-207		 285 / 0.3
115-125-207		 256 / 0.5
115-065-207
 Whole IgG Goat Anti-Mouse IgG, Fcγ subclass 2c specific                   ML*
  (min X Hu, Bov, Rb Sr Prot)		 200 / 1.0
115-005-208		 284 / 0.5
115-485-208		 214 / 0.5
115-095-208		 285 / 0.5
115-115-208		 285 / 0.3
115-135-208		 284 / 0.5
115-495-208		 285 / 0.3
115-125-208		 256 / 0.5
115-065-208
  Whole IgG Goat Anti-Mouse IgG, Fcγ subclass 3 specific                     ML*
  (min X Hu, Bov, Rb Sr Prot)		 200 / 1.0
115-005-209		 284 / 0.5
115-485-209		 214 / 0.5
115-095-209		 285 / 0.5
115-115-209		 285 / 0.3
115-135-209		 284 / 0.5
115-495-209		 285 / 0.3
115-125-209		 256 / 0.5
115-065-209
Antibody Description Unconjugated NEW
DyLight
488
A=493, E=518		 Fluorescein
FITC
A=488, E=520		 Phycoerythrin
R-PE
A=488, E=580		 Allophycocyanin
APC
A=633, 635, E=670		 NEW
DyLight
649*
A=652, E=670

NEW
PerCP
A=488, E=675

 Biotin-SP
(long spacer)
  F(ab')2 Fragment Goat Anti-Mouse IgG, F(ab')2fragment specific           ML*
  (min X Hu, Bov, Hrs Sr Prot)		 € 148 / 1.0 mg
115-006-072		 € 270 / 1.0 mg
115-486-072		 € 213 / 1.0 mg
115-096-072		 € 285 / 1.0 ml
115-116-072		 € 285 / 0.5 ml
115-136-072		 € 270 / 1.0 mg
115-496-072		 € 285 / 0.5 ml
115-126-072		 € 181 / 0.5 ml
115-066-072
  F(ab')2 Fragment Goat Anti-Mouse IgG + IgM (H+L)
  (min X Hu, Bov, Hrs Sr Prot)		 158 / 1.0
115-006-068		 281 / 1.0
115-486-068		 213 / 1.0
115-096-068		     285 / 1.0
115-116-068		 285 / 0.5
115-136-068		 281 / 1.0
115-496-068		 285 / 0.5
115-126-068		 181 / 0.5
115-066-068
  F(ab')2 Fragment Goat Anti-Mouse IgM, μ chain specific                        ML*
  (min X Hu, Bov, Hrs Sr Prot)		 183 / 1.0
115-006-075		 327 / 1.0
115-486-075		 249 / 1.0
115-096-075		 285 / 1.0
115-116-075		 285 / 0.5
115-136-075		 327 / 1.0
115-496-075		 285 / 0.5
115-126-075		 211 / 0.5
115-066-075
  F(ab')2 Fragment Donkey Anti-Rabbit IgG (H+L)                              ML*
  (min X Bov, Ck, Gt, GP, Sy Hms, Hrs, Hu, Ms, Rat, Shp Sr Prot)		 92 / 0.5
711-006-152		 193 / 0.3
711-486-152		 138 / 0.3
711-096-152		 285 / 1.0
711-116-152		 285 / 0.5
711-136-152		 193 / 0.3
711-496-152		 285 / 0.5
711-126-152		 173 / 0.3
711-066-152
 F(ab')2 Fragment Goat Anti-Rabbit IgG (H+L)                                          ML*
  (min X Hu, Ms, Rat Sr Prot)		 147 / 1.0
111-006-144		 256 / 1.0
111-486-144		 194 / 1.0
111-096-144		 285 / 1.0
111-116-144		 285 / 0.5
111-136-144		 256 / 1.0
111-496-144		 285 / 0.5
111-126-144		 160 / 0.5
111-066-144
  F(ab')2 Fragment Donkey Anti-Rat IgG (H+L)                                   ML*
  (min X Bov, Ck, Gt, GP, Sy Hms, Hrs, Hu, Rb, Shp Sr Prot)		 92 / 0.5
712-006-150		 193 / 0.3
712-486-150		 138 / 0.3
712-096-150		 285 / 1.0
712-116-150		 285 / 0.5
712-136-150		 193 / 0.3
712-496-150		 285 / 0.5
712-126-150		 173 / 0.3
712-066-150
  F(ab')2 Fragment Donkey Anti-Rat IgG (H+L)**                                       ML*
  (min X Bov, Ck, Gt, GP, Sy Hms, Hrs, Hu, Ms, Rb, Shp Sr Prot)		 191 / 0.5
712-006-153		 276 / 0.3
712-486-153		 197 / 0.3
712-096-153		 285 / 1.0
712-116-153		 285 / 0.5
712-136-153		 276 / 0.3
712-496-153		 285 / 0.5
712-126-153		 234 / 0.3
712-066-153
  F(ab')2 Fragment Goat Anti-Rat IgG (H+L)                                              ML*
  (min X Hu, Bov, Hrs, Rb Sr Prot)		 148 / 1.0
112-006-143		 256 / 1.0
112-486-143		 194 / 1.0
112-096-143		 285 / 1.0
112-116-143		 285 / 0.5
112-136-143		 256 / 1.0
112-496-143		 285 / 0.5
112-126-143		 160 / 0.5
112-066-143
Antibody Description Unconjugated NEW
DyLight
488
A=493, E=518		 Fluorescein
FITC
A=488, E=520		 Phycoerythrin
R-PE
A=488, E=580		 Allophycocyanin
APC
A=633, 635, E=670		 NEW
DyLight
649*
A=652, E=670

NEW
PerCP
A=488, E=6755

 Biotin-SP
(long spacer)
  F(ab')2 Fragment Goat Anti-Rat IgG, Fcγ fragment specific                     ML*
  (min X Hu, Bov, Hrs Sr Prot)		 € 148 / 1.0 mg
112-006-071		 € 281 / 1.0 mg
112-486-071		 € 213 / 1.0 mg
112-096-071		 € 285 / 1.0 ml
112-116-071		 € 285 / 0.5 ml
112-136-071		 € 281 / 1.0 mg
112-496-071		 € 285 / 0.5 ml
112-126-071		 € 181 / 0.5 ml
112-066-071
  F(ab')2 Fragment Goat Anti-Rat IgG, F(ab')2 fragment specific
  (min X Hu, Bov, Hrs Sr Prot)		 148 / 1.0
112-006-072		 281 / 1.0
112-486-072		 213 / 1.0
112-096-072		 285 / 1.0
112-116-072		 285 / 0.5
112-136-072		 281 / 1.0
112-496-072		 285 / 0.5
112-126-072		 181 / 0.5
112-066-072
  F(ab')2 Fragment Goat Anti-Rat IgM, μ chain specific                              ML*
  (min X Hu, Bov, Hrs Sr Prot)		 183 / 1.0
112-006-075		 327 / 1.0
112-486-075		 249 / 1.0
112-096-075		 285 / 1.0
112-116-075		 285 / 0.5
112-136-075		 327 / 1.0
112-496-075		 285 / 0.5
112-126-075		 211 / 0.5
112-066-075
  F(ab')2 Fragment Donkey Anti-Sheep IgG (H+L)♦                             ML*
  (min X Ck, GP, Sy Hms, Hrs, Hu, Ms, Rb, Rat Sr Prot)		 92 / 0.5
713-006-147		 193 / 0.3
713-486-147		 138 / 0.3
713-096-147		 285 / 1.0
713-116-147		 285 / 0.5
713-136-147		 193 / 0.3
713-496-147		 285 / 0.5
713-126-147		 173 / 0.3
713-066-147
Antibody Description Unconjugated NEW
DyLight
488
A=493, E=518		 Fluorescein
FITC
A=488, E=520		 Phycoerythrin
R-PE
A=488, E=580		 Allophycocyanin
APC
A=633, 635, E=670		 NEW
DyLight
649*
A=652, E=670

NEW
PerCP
A=488, E=675

 Biotin-SP
(long spacer)
  Streptavidin € 76 / 1.0 mg
016-000-084		 € 201 / 1.0 mg
016-480-084		 € 130 / 1.0 mg
016-010-084
DTAF		 € 210 / 1.0 ml
016-110-084
€ 210 / 0.5 ml
016-130-084
 € 201 / 1.0 mg
016-490-084		 € 210 / 0.5 ml
016-120-084		 na
  ChromPure Donkey IgG, F(ab')2 Fragments   173 / 2
017-000-006		 123 / 1.0
017-480-006		 92 / 1.0
017-090-006		     210 / 1.0
017-110-006		 210 / 0.5
017-130-006		 123 / 1.0
017-490-006		 210 / 0.5
017-120-006		 € 112 / 1.0 mg
017-060-006
  ChromPure Goat IgG, F(ab')2 Fragments   173 / 2
005-000-006		 123 / 1.0
005-480-006		 92 / 1.0
005-090-006		     210 / 1.0
005-110-006		 210 / 0.5
005-130-006		 123 / 1.0
005-490-006		 210 / 0.5
005-120-006		 112 / 1.0
005-060-006
  ChromPure Goat IgG, Whole molecule   101 / 10
005-000-003		 67 / 1.0
005-480-003		 53 / 1.0
005-090-003		     210 / 1.0
005-110-003
210 / 0.5
005-130-003
 67 / 1.0
005-490-003		 210 / 0.5
005-120-003		 63 / 1.0
005-060-003
* DyLight 649 and Cy5 are not excited by an argon laser.  They are excited by krypton/argon, red diode, and helium/neon lasers.  Before purchasing these conjugates, please check to be sure your flow cytometer or FACS analyzer is equipped with the appropriate laser to excite DyLight 649 and Cy5.

†

IgY is the original designation for the IgG-like protein found in both serum and egg yolk.

*

ML=Multiple Labeling (see Multiple Labeling for an explanation.).

♦

Warning: BSA and dry milk may contain IgG which will react with this antibody.  Use of BSA and/or dry milk to block or dilute this antibody may increase background and/or reduce secondary antibody titer.

**

Caution: See Selection and Location of Affinity-Purified Antibodies before selecting an antibody adsorbed against closely related species.
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