| 1. |
Incubate with the first primary antibody, in this example rabbit anti-antigen X. Wash. |
2. |
Incubate with an excess of unconjugated Fab anti-body against the host species of
the primary antibody, in this example unconjugated Fab frag-ment goat anti-rabbit IgG (H+L). This presents the rabbit IgG as goat Fab. Wash. |
3. |
Incubate with Probe 1-conjugated tertiary antibody directed against the host species of the Fab antibody. The tertiary
antibody must not recognize the host species of either the primary antibodies or the second secondary antibody, in this example DyLight 488-mouse anti-goat IgG (H+L) (min X Ms, Hu, Rb Sr Prot). Wash.
|
