Both of these rhodamine derivatives have different absorption (550 nm and 596 nm. respectively) and emission (570 nm and 620 nm,
respectively) maxima (Figure 2). Although TRITC has been used most commonly with FITC for double labeling, better color
separation is achieved by using Rhodamine Red-X (an improved form of Lissamine Rhodamine, see following technical information
on RRX) or Texas Red (Titus et al., J. Immunol. Methods. 1982. 50, 193). However, the use of Texas Red
may lead to slightly higher background staining (Wessendorf and Brelje, Histochemistry. 1992. 98, 81). For double labeling
in flow cytometry, phycoerythrin (instead of Rhodamine) conjugates (see product listings) are recommended for use with FITC
since both can be excited by a single wavelength (488 nm) of light.
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