The selection of fluorophores depends on:

1. Instrument set-up. Examples include availability of light sources, filter sets, and detection systems.
   
2. Degree of color separation desired for multiple labeling. For example, to achieve good color separation from DyLight 488, choose a longer wavelength-emitting fluorophore such as DyLight 594 or DyLight 649 rather than DyLight 549.
   
3. Sensitivity required. For example, DyLight 488, DyLight 549, DyLight 594, and DyLight 649 are brighter than FITC, Cy2, TRITC, RRX, Texas Red, and Cy5.

• NEW  DyLight fluorescent dyes

• Aminomethylcoumarin Acetate (AMCA)

• Fluorescein Isothiocyanate (FITC)

• Cyanine dyes (Cy2, Cy3, and Cy5)

• Tetramethyl Rhodamine Isothiocyanate (TRITC), Rhodamine Red-X (RRX),
   and Texas Red (TR)

• Secondary Antibody Conjugates for Flow Cytometry

• NEW  Text Files for all fluorescent dye conjugates to compare excitation
  and emission spectra

Other Probes

• Biotin-SP (long-spacer)

• Enzymes